Biocompatibility of chitosan-coated iron oxide nanoparticles with osteoblast cells.

摘要: Background Bone disorders (including osteoporosis, loosening of a prosthesis, and bone infections) are great concern to the medical community difficult cure. Therapies available treat such diseases, but all have drawbacks not specifically targeted site disease. Chitosan is widely used in biomedical community, including for orthopedic applications. The aim present study was coat chitosan onto iron oxide nanoparticles determine its effect on proliferation differentiation osteoblasts. Methods Nanoparticles were characterized using transmission electron microscopy, dynamic light scattering, x-ray diffraction, zeta potential, vibrating sample magnetometry. Uptake by osteoblasts studied microscopy Prussian blue staining. Viability measured presence uncoated magnetic or those coated with chitosan. Lactate dehydrogenase, alkaline phosphatase, total protein synthesis, extracellular calcium deposition nanoparticles. Results Chitosan-coated enhanced osteoblast proliferation, decreased cell membrane damage, promoted differentiation, as indicated an increase phosphatase deposition. showed good compatibility Conclusion Further research necessary optimize treatment