Characterization of rotavirus and norovirus strains: a 6-year study (2004-2009)

摘要: OBJECTIVE: To monitor rotavirus (RV) and norovirus (NoV) infections in hospitalized children < 5 years with acute gastroenteritis the state of Sao Paulo, Brazil, during a 6-year period (2004- 2009). METHODS: This retrospective study was conducted 61 medical centers convenient surveillance fecal specimens, investigated by enzyme-linked immunosorbent assay, sodium dodecyl sulfate polyacrylamide gel electrophoresis, reverse polymerase chain reaction sequencing to genotype characterization. RESULTS: RV NoV were detected 29.6% (144∕487) 29.2% (26/89) samples, respectively. The most frequent genotypes G9P[8] 2004; G1P[8] 2005; 2006; G2P[4] 2007, 2008, 2009. Detection rate declined from 36.3% (33∕91) 2004 4.2% (4/95) genogroup GII found 61.6% (16/26) GI 11.5% (3/26). Mixed NoV-RV observed 2.2% (2/89) involving GI+G9P[8] GI+G2P[4] strains. CONCLUSIONS: Genotype distribution varied according collection year, accompanied reduction detection rate. Use vaccine requires implementation post-marketing strain diversity its efficacy against possible new emerging genotypes. NoVs have been increasingly identified as relevant etiological agents among play an important role viral etiology pediatric Paulo.