Characterization of acetylcholinesterases of acaricide-resistant and susceptible strains of the cattle tick Boophilus microplus (Can.)
作者： James Nolan , H.J. Schnitzerling
DOI: 10.1016/0048-3575(75)90073-5
关键词: Tick 、 Microbiology 、 Trichostrongylus 、 Acaricide 、 Substrate (chemistry) 、 Nematode 、 Acetylthiocholine 、 Lucilia cuprina 、 Enzyme 、 Biology 、 Agronomy and Crop Science 、 Health, Toxicology and Mutagenesis 、 General Medicine
摘要: Abstract The critical acetylcholinesterase component, that is responsible for the resistance mechanism of decreased sensitivity to inhibitor in cattle tick Boophilus microplus (Can.), has been isolated from organophosphorus-susceptible Yeerongpilly and resistant Biarra Ridgelands strains. With substrate acetylthiocholine this enzyme component gave a pH activity profile similar all Optimal concentration was 1.73.10 −2 M compared 2.70.10 −4 other Computed Km values were 1.98.10 −5 , 3.65.10 6.43.10 −6 Yeerongpilly, Biarra, strains, respectively, while corresponding V max figures 336, 135, 21 μmoles hydrolyzed per gram larvae hour. These results are discussed relation with parameters derived bovine erythrocytes, sheep blowfly Lucilia cuprina nematode Trichostrongylus colubtiformis .
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