Membrane trafficking: Adaptor waves at the Golgi

摘要: During membrane trafficking between the trans Golgi network (TGN) and endosomes, clathrin-coated vesicles are formed through concerted action of adaptor proteins that link clathrin, cargo. Here, Daboussi et al. use live cell imaging super-resolution microscopy to dissect relationship Ap1 Gga2 in budding yeast. They find these recruited TGN two waves during coat assembly, with arriving first. Levels phosphoinositide PtdIns4P, which mediates localization adaptors TGN, important for ensuring proper rate progression. In addition, recruits Pik1, a kinase generates direct interaction. Thus, this mechanism may provide means generating adaptor-specific coats coated distinct target destinations.