Genomic organization and regulatory elements of the rat latexin gene, which is expressed in a cell type-specific manner in both central and peripheral nervous systems

摘要: Abstract Latexin, a carboxypeptidase A inhibitor, is expressed in cell type-specific manner both central and peripheral nervous systems the rat. In neocortex, specific subpopulation of neurons layers V VI expresses latexin. primary sensory ganglia, expression restricted to smaller diameter neurons. As first step clarify regulatory mechanisms underlying latexin, we have determined organization rat latexin gene analyzed its elements. The spans approximately 5.8 kb, consists six exons five introns. Three transcription initiation sites were mapped. upstream region lacks typical TATA or CAAT boxes but has several GC-rich sites. To assess promoter activity, luciferase reporter fused 5′-flanking (6.4 kb) was transiently transfected into lines. Luciferase activity 2–8 times higher latexin-expressing cells (PC12) than non-expressing (NS20 L6). Deletion analysis with PC12 revealed that core located between nucleotide positions −261 −201 relative codon. Nerve growth factor (NGF)-responsive element(s) −518 −262, which AP-1, AP-2 NF-κB binding are found. Furthermore, demonstrate 1.3 kb genomic fragment containing intron transcriptional enhancing cells. These results suggest up downstream elements involved control