Comparative Quantitation of Aberrant Glycoforms by Lectin-Based Glycoprotein Enrichment Coupled with Multiple-Reaction Monitoring Mass Spectrometry
作者： Yeong Hee Ahn , Yong-Sam Kim , Eun Sun Ji , Ji Yeon Lee , Ji-Ae Jung
DOI: 10.1021/AC1001965
关键词: Matrix metalloproteinase 、 Protein tyrosine phosphatase 、 Molecular biology 、 Mass spectrometry 、 Lectin 、 Selected reaction monitoring 、 Tissue inhibitor of metalloproteinase 、 TIMP1 、 Glycoprotein 、 Chemistry
摘要: Lectin enrichment-coupled multiple-reaction monitoring (MRM) mass spectrometry was employed to quantitatively monitor the variation of aberrant glycoforms produced under pathological states. For this, tissue inhibitor metalloproteinase 1 (TIMP1) and protein tyrosine phosphatase κ (PTPκ), previously known target proteins for N-acetylglucosaminyltransferase-V (GnT-V), were enriched by phytohemagglutinin-L4 (L-PHA) lectin comparatively analyzed in conditioned medium WiDr colon cancer cell line its GnT-V-overexpressing transfectant cells. Enriched digested, resultant peptides quantified MRM analysis. quantitation data L-PHA-enriched samples revealed that abundance TIMP1 PTPκ greatly increased (11.7- 16.5-fold, respectively) GnT-V-treated cells compared control cells, although total slightly diff...
acs.org
core.ac.uk 
sci-hub.se 参考文章(33)
M Pierce, J Arango, Rous sarcoma virus-transformed baby hamster kidney cells express higher levels of asparagine-linked tri- and tetraantennary glycopeptides containing [GlcNAc-beta (1,6)Man-alpha (1,6)Man] and poly-N-acetyllactosamine sequences than baby hamster kidney cells. Journal of Biological Chemistry. ,vol. 261, pp. 10772- 10777 ,(1986) , 10.1016/S0021-9258(18)67453-8
R D Cummings, S Kornfeld, Characterization of the structural determinants required for the high affinity interaction of asparagine-linked oligosaccharides with immobilized Phaseolus vulgaris leukoagglutinating and erythroagglutinating lectins. Journal of Biological Chemistry. ,vol. 257, pp. 11230- 11234 ,(1982) , 10.1016/S0021-9258(18)33746-3
Tasneem H. Patwa, Jia Zhao, Michelle A. Anderson, Diane M. Simeone, David M. Lubman, Screening of glycosylation patterns in serum using natural glycoprotein microarrays and multi-lectin fluorescence detection. Analytical Chemistry. ,vol. 78, pp. 6411- 6421 ,(2006) , 10.1021/AC060726Z
Torben F. Orntoft, Else Marie Vestergaard, Clinical aspects of altered glycosylation of glycoproteins in cancer. Electrophoresis. ,vol. 20, pp. 362- 371 ,(1999) , 10.1002/(SICI)1522-2683(19990201)20:2<362::AID-ELPS362>3.0.CO;2-V
Michael A. Kuzyk, Derek Smith, Juncong Yang, Tyra J. Cross, Angela M. Jackson, Darryl B. Hardie, N. Leigh Anderson, Christoph H. Borchers, Multiple Reaction Monitoring-based, Multiplexed, Absolute Quantitation of 45 Proteins in Human Plasma Molecular & Cellular Proteomics. ,vol. 8, pp. 1860- 1877 ,(2009) , 10.1074/MCP.M800540-MCP200
Thea D Tlsty, Stromal cells can contribute oncogenic signals Seminars in Cancer Biology. ,vol. 11, pp. 97- 104 ,(2001) , 10.1006/SCBI.2000.0361
Kathleen J. Green, Cory L. Simpson, Desmosomes: New Perspectives on a Classic Journal of Investigative Dermatology. ,vol. 127, pp. 2499- 2515 ,(2007) , 10.1038/SJ.JID.5701015
Yi-Mi Wu, D. David Nowack, Gilbert S. Omenn, Brian B. Haab, Mucin glycosylation is altered by pro-inflammatory signaling in pancreatic-cancer cells. Journal of Proteome Research. ,vol. 8, pp. 1876- 1886 ,(2009) , 10.1021/PR8008379
Karen L. Abbott, Kazuhiro Aoki, Jae-Min Lim, Mindy Porterfield, Rachelle Johnson, Ruth M. O’Regan, Lance Wells, Michael Tiemeyer, Michael Pierce, Targeted glycoproteomic identification of biomarkers for human breast carcinoma. Journal of Proteome Research. ,vol. 7, pp. 1470- 1480 ,(2008) , 10.1021/PR700792G
Mikala Egeblad, Zena Werb, New functions for the matrix metalloproteinases in cancer progression Nature Reviews Cancer. ,vol. 2, pp. 161- 174 ,(2002) , 10.1038/NRC745