Phagocytosis of liposomes by macrophages: intracellular fate of liposomal malaria antigen

摘要: Abstract Liposomes containing a synthetic recombinant protein were phagocytosed by macrophages, and the internalized was recycled to cell surfaces where it detected enzyme-linked immunosorbent assay. The transit time of liposome-encapsulated from initial phagocytosis liposomes appearance on macrophages determined pulse-chase experiments. pulsed with chased empty liposomes, vice versa. amount rate antigen expression at depended quantity encapsulated ingested macrophages. Although rapidly taken up antigenically expressed for prolonged period (at least 24 h) surface. visualized inside vacuoles in immunogold electron microscopy. accumulated along peripheries many them apparently remained intact long (> 6 h). However, nonliposomal free also cytoplasm surrounding these vacuoles, concluded that probably en route macrophage Exposure cells ammonium chloride did not inhibit liposomal antigenic epitopes surface, this suggests surface pH-sensitive phenomenon. There no significant effect adjuvant, lipid A, or extent protein.