Genes up- or down-regulated by high calcium medium in parathyroid tissue explants from patients with primary hyperparathyroidism.

摘要: To investigate genes modulated in the parathyroid glands by calcium, expression levels of mRNA for all expressed tissue explants (PTEs) obtained from patients with primary hyperparathyroidism (I°-HPT) were analyzed oligo-DNA microarray. PTEs 4 I°-HPT precultured normocalcemic medium (Ca++ 1.0-1.1 mM) 7 days and then cultured hypocalcemic 0.60 or hypercalcemic 1.60 containing 4mg/dl phosphate an additional days. As expected, PTH chromogranin A decreased to less than 50% when compared those medium. Furthermore, microarray analyses revealed that up-regulated more 2-fold 30 down-regulated 1/2 PTEs. Interestingly, 9 these (up-regulated genes: chemokine ligand 8, multiple C2 domain transmembrane region protein 1; matrix metallopeptidase-9, B-box SPRY domain-containing protein, nitric oxide synthase 2A, PTH, cartilage acidic 1, A, fibrin 1) involved calcium metabolism calcium-signaling pathways tissue. However, level α-klotho was variable, it not constantly under present experimental conditions. Although possible use normal tissue, this is first reported study have investigated human adenomas are high concentration organ culture.