Fluoride Modulates Parathyroid Hormone Secretion in vivo and in vitro.

摘要: The study objective was to investigate the effects of fluoride on intact parathyroid hormone (iPTH) secretion. Thyro-parathyroid complexes (TPC) from C3H (n = 18) and B6 mice were cultured in Ca²⁺-optimized medium. TPC treated with 0, 250, or 500 µM NaF for 24 h secreted iPTH assayed by ELISA. 78) gavaged once distilled (0.001 mg [F⁻]/g body weight) water. At serial time points (0.5-96 h) serum iPTH, fluoride, total calcium, phosphorus, magnesium levels determined. Expression genes involved mineral regulation via bone-parathyroid-kidney (BPK) axis, such as (Pth), calcium-sensing receptor (Casr), vitamin D (Vdr), hormone-like (Pthlh), fibroblast growth factor 23 (Fgf23), α-Klotho (αKlotho), 1c (Fgf1rc), tumor necrosis 11 (Tnfs11), 1 (Pth1r), solute carrier family 34 member (Slc34a1), 9 3 regulator (Slc9a3r1), chloride channel 5 (Clcn5), PDZ domain-containing (Pdzk1), determined TPC, humeri, kidneys at h. An vitro decrease seen (p < 0.001). In vivo peaked 0.5 both 0.002) 0.01). C3H, decreased 0.0001), returning baseline 48 B6, increased 12 0.001), Serum did not change significantly. Pth, Casr,αKlotho,Fgf1rc,Vdr, Pthlh significantly upregulated compared B6. conclusion, equivalent between 2 mouse strains. However, demonstrated an early strain-dependent effect secretion vivo. Both strains differences expression BPK suggesting a possible role physiologic handling fluoride.