Prediction and verification of miRNA expression in human and rat retinas.

摘要: PURPOSE. MicroRNAs (miRNAs) play a global role in regulating gene expression and have important tissue-specific functions. Little is known about their the retina. The purpose of this study was to establish retinal those miRNAs predicted target genes involved vision. METHODS. potentially targeting "retinal" genes, as defined by pattern implication disease, were using published algorithm (TargetScan; Envisioneering Medical Technologies, St. Louis, MO). presence candidate human rat RNA assessed RT-PCR. cDNA levels for each miRNA determined quantitative PCR. ability discriminate between varying single nucleotide assessed. activity miR-124 miR-29 against sites Rdh 10 Impdh1 tested cotransfection mimics luciferase reporter plasmids. RESULTS. Sixty-seven one or more 320 listed herein. All 11 expressed retina, including miR-7, miR-124, miR135a, miR135b. Relative individual similar rats humans. 3'UTR, which contains site, mediated inhibition cell culture. CONCLUSIONS. Many likely regulate function are present Conservation patterns from humans supports evidence other tissues that disruption cause range visual abnormalities.