MicroRNA-215 Regulates Fibroblast Function: Insights from a Human Fibrotic Disease

摘要: MicroRNAs are implicated in the regulation of gene expression via various mechanisms health and disease, including fibrotic processes. Pterygium is an ocular surface condition characterized by abnormal fibroblast proliferation matrix deposition. We aimed to investigate role microRNAs pterygium understand relevant cellular molecular mechanisms. To achieve this objective, a combination approaches using surgically excised paired human conjunctival tissues as well cultured primary cells from tissue explants were evaluated. Fibroblast dysfunction has been shown play central pathology. Here we show that miR-215, among few others, was down-regulated (2-fold) compared control, consistent microarray, real-time PCR fluorescent in-situ hybridization. The effects increased miR-215 investigated adding exogenous fibroblasts, showed decrease cell but no significant apoptosis control. Further cycle analysis depressed progression at G1/S G2/M. A related transcripts downregulated (2.2-4.5-fold) on addition miR-215: Mcm3, Dicer1, Cdc25A, Ick, Trip13 Mcm10. Theoretic binding energies used predict targets luciferase reporter studies confirmed Mcm10 Cdc25A direct targets. In summary, mir-215 could inhibiting conjunctiva. Dampening result cycling proliferation, with possibly fibroblastic production matrix, inducing formation.