Evolutionary Diversification of Eukaryotic DNA Replication Machinery

摘要: DNA replication research to date has focused on model organisms such as the vertebrate Xenopus laevis and yeast species Saccharomyces cerevisiae Schizosaccharomyces pombe. However, animals fungi both belong Opisthokonta, one of about six eukaryotic phylogenetic ‘supergroups’, therefore represent only a fraction diversity. To explore evolutionary diversification machinery bioinformatic approach was used investigate presence or absence yeast/animal replisome components in other taxa. A comparative genomic survey undertaken 59 proteins diverse range 36 eukaryotes from all supergroups. Twenty-three including Mcm2–7, Cdc45, RPA1, primase, some polymerase subunits, RFC1–5, PCNA Fen1 are present examined. further 20 supergroups, although not necessarily every species: with exception RNase H2B fork protection complex component Timeless/Tof1, these members anciently derived paralogous families ORC, MCM, GINS RPA. Together form set 43 that must have been last common ancestor (LCEA). This minimal LCEA is significantly more than related Archaea, indicating events duplications genes lineage which parallel early evolution cellular features.