Genetic and antigenic variability of HIV type 1 in Brazil.

摘要: Six Brazilian strains of human immunodeficiency virus type 1 (HIV-1) were isolated from infected individuals residing in different regions Brazil between 1987 and 1989. Phylogenetic analysis based on an 860-base pair env fragment, including V3, V4, V5, the beginning gp41, classified significantly genotype B, with interhost distances 5.9 13.1% (mean value, 10%). Amino acid sequence V3 loop revealed that three contained North American/European GPGR motif as tip whereas other proline (P) was substituted by tryptophan (W), methionine (M), or phenylalanine (F). A consensus peptide, Bra-cons, designed containing GWGR loop. Serological reactivity to Bra-cons peptide peptides (MN, SF2, HBX2, RF, MAL, ELI, Z6, a Cote d'Ivoire CI-cons) compared for 114 HIV-1-positive sera Rio de Janeiro. Sixty-nine (60.5%) reacted belonging which 10 also (n = 7) D 3). Eighteen (15.8%) had binding antibodies peptide. high number 43; 37.7%) no any tested. This result suggests HIV-1 variants aberrant loops may circulate