Sizing of the human T cell receptor alpha locus and detection of a large deletion in the Molt-4 cell line.
作者： MS Roth , FS Collins , D Ginsburg
DOI: 10.1182/BLOOD.V71.6.1744.1744
关键词: CD8 、 Biology 、 Gene 、 Molecular biology 、 DNA 、 T cell 、 T-cell receptor 、 Locus (genetics) 、 Gel electrophoresis 、 Restriction fragment
摘要: The human T cell receptor alpha (TCR-alpha) chain gene consists of discontinuous DNA segments encoding multiple variable (V), joining (J), and one constant (C) region. Unlike other immunoglobulin or TCR genes, however, the TCR-alpha locus exhibits an unusual dispersal J that occupy a region greater than 50 kilobases (kb) upstream C region, with exact size still unknown. We report here study genetic by using pulsed-field gel electrophoresis (PFGE), which permits separation large fragments. Analysis prepared from normal peripheral blood mononuclear cells, endothelial B line demonstrates both V sequences are contained within single 400-kb SfiI restriction fragment. PFGE analysis Molt 4 suggests 600-kb deletion involving TCR- gene.
ashpublications.org
sci-hub.se 参考文章(18)
Michael E Williams, Donald J Innes Jr, Michael J Borowitz, Mark A Lovell, Steven H Swerdlow, Paul E Hurtubise, Russell K Brynes, Wing C Chan, Gerald E Byrne Jr, Clarence C Whitcomb, Christopher Y Thomas, Immunoglobulin and T cell receptor gene rearrangements in human lymphoma and leukemia. Blood. ,vol. 69, pp. 79- 86 ,(1987) , 10.1182/BLOOD.V69.1.79.79
Cassandra L Smith, Simon K Lawrance, Gerald A Gillespie, Charles R Cantor, Sherman M Weissman, Francis S Collins, Strategies for mapping and cloning macroregions of mammalian genomes. Methods in Enzymology. ,vol. 151, pp. 461- 489 ,(1987) , 10.1016/S0076-6879(87)51038-2
Jeffrey M. Leiden, John D. Fraser, Jack L. Strominger, The complete primary structure of the T-cell receptor genes from an alloreactive cytotoxic human T-lymphocyte clone. Immunogenetics. ,vol. 24, pp. 17- 23 ,(1986) , 10.1007/BF00372293
N. C. Dracopoli, A. N. Houghton, L. J. Old, Loss of polymorphic restriction fragments in malignant melanoma: implications for tumor heterogeneity Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 1470- 1474 ,(1985) , 10.1073/PNAS.82.5.1470
Y. Yanagi, A. Chan, B. Chin, M. Minden, T. W. Mak, Analysis of cDNA clones specific for human T cells and the alpha and beta chains of the T-cell receptor heterodimer from a human T-cell line. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 3430- 3434 ,(1985) , 10.1073/PNAS.82.10.3430
H. D. Royer, D. Ramarli, O. Acuto, T. J. Campen, E. L. Reinherz, Genes encoding the T-cell receptor alpha and beta subunits are transcribed in an ordered manner during intrathymic ontogeny Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 5510- 5514 ,(1985) , 10.1073/PNAS.82.16.5510
F. Collins, M. Drumm, J. Cole, W. Lockwood, G. Vande Woude, M. Iannuzzi, Construction of a general human chromosome jumping library, with application to cystic fibrosis Science. ,vol. 235, pp. 1046- 1049 ,(1987) , 10.1126/SCIENCE.2950591
R N Sangster, J Minowada, N Suciu-Foca, M Minden, T W Mak, Rearrangement and expression of the alpha, beta, and gamma chain T cell receptor genes in human thymic leukemia cells and functional T cells. Journal of Experimental Medicine. ,vol. 163, pp. 1491- 1508 ,(1986) , 10.1084/JEM.163.6.1491
B. Toyonaga, Y. Yoshikai, V. Vadasz, B. Chin, T. W. Mak, Organization and sequences of the diversity, joining, and constant region genes of the human T-cell receptor beta chain. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 8624- 8628 ,(1985) , 10.1073/PNAS.82.24.8624
David C. Schwartz, Charles R. Cantor, Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis Cell. ,vol. 37, pp. 67- 75 ,(1984) , 10.1016/0092-8674(84)90301-5