5-Hydroxytryptamine-stimulated calcium mobilization in cultured canine tracheal smooth muscle cells
作者： C.M. Yang , J-T. Hsieh , Y-L. Yo , R. Ong , H-L. Tsao
DOI: 10.1016/0143-4160(94)90022-1
关键词: Extracellular 、 Diltiazem 、 Calcium 、 EGTA 、 Receptor 、 Channel blocker 、 Verapamil 、 Internal medicine 、 Ketanserin 、 Biology 、 Endocrinology 、 Cell biology 、 Physiology 、 Molecular biology
摘要: Abstract 5-Hydroxytryptamine (5-HT)-induced increase of intracellular Ca 2+ concentration ([Ca ] i ) was monitored in cultured canine tracheal smooth muscle cells (TSMCs) using a fluorescent indicator Fura-2. Stimulation TSMCs by 5-HT produced an initial transient peak followed sustained, concentration-dependent elevation [Ca . The log (EC 50 values for the and sustained plateau responses were −7.43 −7.60 M, respectively. 1A 3 receptor antagonists, NAN-190 metoclopramide, inhibited 5-HT-stimulated with pK B 6.3 6.2, respectively, indicating that receptors mediating signal had low affinity these antagonists. In contrast, 2A ketanserin mianserin, high antagonizing changes response to 8.3 8.3, dependent on presence extracellular Removal addition 2 mM EGTA during phase caused rapid decline resting level. absence , only observed which then declined level; could be evoked 1.8 continued 5-HT. influx required since -channel blockers, diltiazem, verapamil, Ni decreased both These blockers also when applied phase. conclusion, findings indicate stimulated acting is due release from internal stores, external into cells. partially involves diltiazem verapamil sensitive channel.
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