Ontogenetic expression and 17β-estradiol regulation of immune-related genes in early life stages of Japanese medaka (Oryzias latipes)

摘要: Abstract Accumulating evidence suggests that environmental endocrine disrupting chemicals (EDCs) may exert adverse effects on aquatic organisms via the modulation of immune competence in addition to system. However, date, most studies have been undertaken only biochemical and histopathological endpoints, few addressed role response gene transcript abundance estrogen. In present study, ontogenetic expression immune-related genes, including three complement components (C3-1, C3-2 Bf/C2), two cytokines (IL-21 type I IFN [IFN]), lysozyme (LZM), novel immune-type receptor (NITR-18), Ikaros (IK) ceruloplasmin (CP) were characterized during different developmental periods (from 0 28 d post-hatch [dph]) Japanese medaka. Furthermore, responses these genes natural estrogen (i.e., 17β-estradiol [E2]) evaluated. E2 exposure at sublethal concentrations (0.1–10 μg/L) down-regulated C3-1, C3-2, Bf/C2, LZM CP, while up-regulating IL-21, IFN, NITR-18 IK. The results demonstrate a very trend fish larvae exposed when compared with changes control, suggesting estrogenic activities interfere thus potentially influence susceptibility opportunistic infections. These findings confirm ability exogenous estrogens elicit expression, broaden our understanding about mechanisms underlying actions EDCs. addition, profiles can be developed for use as biomarkers future immunotoxicological studies.