Occurrence of foodborne pathogenic bacteria in retail prepackaged portions of marine fish in Spain

摘要: Aims:  To survey the presence of indigenous and nonindigenous foodborne bacterial pathogens in displayed prepacked portions fresh marine fish. Methods Results:  A 50 different samples fish (conger, swordfish, sole, grouper whiting) was conducted over a period 5 months. Trays fillets steaks were obtained at retail level tested for pathogens. Vibrio cholerae Salmonella not detected. Two (4%) yielded strains carrying DNA fragment specific parahaemolyticus, but resulted negative to PCR amplification virulence-related tdh gene. Levels motile Aeromonas ranging from 2·29 7·20 log CFU g−1 found 31 (62%) samples. All positive hlyA gene 38 both aerA genes, which may contribute diarrhoea-related virulence. The incidence Listeria monocytogenes 10%. Staphylococcus aureus lower than 2 log CFU g−1 15 (30%) Numbers presumptive Clostridium perfringens 1·82 ± 0·22 4·26 ± 1·25 log CFU g−1 detected 42 (84%) Edwardsiella tarda two fillets. Conclusions:  Displayed raw carried bacteria that can cause disease. risk posed by when properly cooked is low, high destined be consumed raw, undercooked or very lightly processed. Significance Impact Study:  This study revealed sold Spain could source Improvements handling processing are needed minimize prevalence pathogenic bacteria.