In vivo FLUORESCENCE KINETICS OF PHTHALOCYANINES IN A SKIN‐FOLD OBSERVATION CHAMBER MODEL: ROLE OF CENTRAL METAL ION AND DEGREE OF SULFONATION

摘要: — The fluorescence pharmacokinetics of a series metallosulfophthalocyanines, chelated with either aluminum or zinc and sulfonated to different degrees, was studied by measurements in vivo. Dyes were administered systemically female WAG/RIJ rats an isogeneic mammary carcinoma transplanted into the subcutis transparent observation chamber located on their backs. Following intravenous injection 2.5 μmol/ kg dye, dynamics observed up 7 h postinjection. phthalocyanines excited at 610 nm power density 0.1 mW/cm2 without causing photodynamic damage vasculature. Fluorescence detected above 665 using imaging system based image intensifier. Dye retention blood vessels tumor tissue expressed as ratios relative signal surrounding subcutaneous tissue. Phthalocyanincs gave highest tumor-over-subcutis value 4. complexes exhibited vascular-over-subcutis maximum values exceeding 6. They also displayed longest times vascular well over h. Overall, decreasing degree sulfonation metallophthalocyanines results lower tumor-over-normal ratios, furthermore aluminum-based dyes seem superior localizers zinc-based dyes. advantages porphyrins respect localization therapy are discussed.