Apoptotic pathways and stemness in the colorectal epithelium and lamina propria mucosae during the human embryogenesis and carcinogenesis.

摘要: Abstract Aim Programmed cell death is essential both during normal organ development and carcinogenesis. In this study we immunohistochemically analyzed different pathways of in 11 human conceptuses 5th-10th-weeks old, 10 low high grade colorectal carcinomas (CRC), colon samples by using markers for apoptosis (caspase-3, AIF, TUNEL), proliferation (Ki-67) stemness (Oct-4). Results Between the 5th 10th week development, caspase-3 AIF showed moderate-to-strong expression developing gut wall. During number caspase-3-reactive cells decreased, while increased. While healthy control CRC moderate their was strong. Tumor tissues displayed significantly higher positive than controls. Occasionally, co-expressing characterized dying cells. colon, Oct-4 Ki-67 expression, some co-expressed markers. Their decreased epithelium increased connective tissue later development. Healthy mild reactivity. low-grade moderate, high-grade Although TUNEL occasionally all samples, grades contained that were only. Conclusion Our revealed two parallel death, with characteristic increase AIF-mediated when compared to caspase-3, presence These finding might be considered as important diagnostic, survival therapy predictors.