A targeted gain of function screen in the embryonic CNS of Drosophila.
作者： Vicki L McGovern , Christina A Pacak , Stephen T Sewell , Michelle L Turski , Mark A Seeger
DOI: 10.1016/S0925-4773(03)00159-X
关键词: Axon guidance 、 Genetics 、 Krüppel 、 Axon 、 Zinc finger 、 Transcription factor 、 Embryonic stem cell 、 Gene 、 Biology 、 Hepatocyte nuclear factor 4
摘要: In order to identify genes involved in the development of central nervous system (CNS) we have undertaken a gain function screen embryonic CNS Drosophila. Transposable P-elements and UAS/GAL4 were used initiate transcription pan-neural pattern using scaGAL4. Over 4100 individual P-element insertion lines screened with monoclonal antibodies BP102 1D4 visualize axon pathways. Twenty-five insertions corresponding 18 resulted aberrant pathfinding when misexpressed Genes guidance, patterning, cell cycle regulation isolated. addition, identified several zinc finger factors not previously implicated guidance or development. This group includes Squeeze, Kruppel homolog-1, Hepatocyte nuclear factor 4, two uncharacterized genes, CG11966 CG9650. Calnexin99A, putative molecular chaperone, was isolated as well.
elsevier.com
sci-hub.se 参考文章(65)
W. Zhong, F.M. Sladek, J.E. Darnell, The expression pattern of a Drosophila homolog to the mouse transcription factor HNF-4 suggests a determinative role in gut formation. The EMBO Journal. ,vol. 12, pp. 537- 544 ,(1993) , 10.1002/J.1460-2075.1993.TB05685.X
E Bier, J W O'Neill, Double-label in situ hybridization using biotin and digoxigenin-tagged RNA probes. BioTechniques. ,vol. 17, pp. 870- 875 ,(1994)
José A. Campos-Ortega, Volker Hartenstein, The embryonic development of Drosophila melanogaster Springer Berlin Heidelberg. ,(1985) , 10.1007/978-3-662-02454-6
Shingo Yoshikawa, Joshua L. Bonkowsky, Michelle Kokel, Stanley Shyn, John B. Thomas, The Derailed Guidance Receptor Does Not Require Kinase Activity In Vivo The Journal of Neuroscience. ,vol. 21, ,(2001) , 10.1523/JNEUROSCI.21-01-J0002.2001
Peter Tsai, Yuchun He, Margit Pál, Hugo J. Bellen, David M. Glover, Péter Maróy, Sergei N. Prokopenko, Péter Deák, Péter Deák, Adi Salzberg, P-element insertion alleles of essential genes on the third chromosome of Drosophila melanogaster: mutations affecting embryonic PNS development. Genetics. ,vol. 147, pp. 1723- 1741 ,(1997) , 10.1093/GENETICS/147.4.1723
Gerald M. Rubin, Audrey M. Huang, A Misexpression Screen Identifies Genes That Can Modulate RAS1 Pathway Signaling in Drosophila melanogaster Genetics. ,vol. 156, pp. 1219- 1230 ,(2000) , 10.1093/GENETICS/156.3.1219
Barry Ganetzky, Chang-Sook Hong, Molecular Characterization of Neurally Expressing Genes in the para Sodium Channel Gene Cluster of Drosophila Genetics. ,vol. 142, pp. 879- 892 ,(1996) , 10.1093/GENETICS/142.3.879
Gary H. Karpen, Kenneth W. Dobie, Cameron D. Kennedy, Ryan W. Patterson, Vivienne M. Velasco, Juliani Weko, Tory L. McGrath, Identification of chromosome inheritance modifiers in Drosophila melanogaster. Genetics. ,vol. 157, pp. 1623- 1637 ,(2001) , 10.1093/GENETICS/157.4.1623
Barry Condron, Gene Expression Is Required for Correct Axon Guidance Current Biology. ,vol. 12, pp. 1665- 1669 ,(2002) , 10.1016/S0960-9822(02)01185-5
S. Christodoulou, A.E. Lockyer, J.M. Foster, J.D. Hoheisel, D.B. Roberts, Nucleotide sequence of a Drosophila melanogaster cDNA encoding a calnexin homologue Gene. ,vol. 191, pp. 143- 148 ,(1997) , 10.1016/S0378-1119(97)00025-5