Incorporation of the Green Fluorescent Protein into the Herpes Simplex Virus Type 1 Capsid
作者： Prashant Desai , Stanley Person
DOI: 10.1128/JVI.72.9.7563-7568.1998
关键词: Fusion protein 、 Herpes simplex virus 、 Virus 、 Open reading frame 、 Molecular biology 、 Protein biosynthesis 、 Biology 、 Green fluorescent protein 、 Capsid 、 Aequorea victoria
摘要: The herpes simplex virus type 1 (HSV-1) UL35 open reading frame (ORF) encodes a 12-kDa capsid protein designated VP26. VP26 is located on the outer surface of specifically tips hexons that constitute shell. bioluminescent jellyfish (Aequorea victoria) green fluorescent (GFP) was fused in with ORF to generate VP26-GFP fusion protein. This and localized distinct regions within nuclei transfected cells following infection wild-type virus. marker introduced into HSV-1 (KOS) genome resulting recombinant plaques were fluorescent. A virus, K26GFP, isolated purified shown grow as well cell culture. An analysis intranuclear capsids formed K26GFP-infected revealed incorporated A, B, C capsids. Furthermore, virion particle judged by fluorescence-activated sorter (FACS) infected absence de novo synthesis. Cells K26GFP exhibited punctate nuclear fluorescence at early times replication cycle. At later during generalized cytoplasmic fluorescence, including membranes, observed, confirming visually mature virions.
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