Quantitative PSA mRNA determination in blood: a biochemical tool for scoring localized prostate cancer.
作者： Carlo-Federico Zambon , Daniela Basso , Tommaso Prayer-Galetti , Filippo Navaglia , Michela Fasolo
DOI: 10.1016/J.CLINBIOCHEM.2006.02.001
关键词: Prostate cancer 、 Messenger RNA 、 Oncology 、 Prostate 、 Grading (tumors) 、 Internal medicine 、 Sensitive index 、 Free psa 、 Hyperplasia 、 Medicine 、 Peripheral blood mononuclear cell
摘要: Abstract Objectives: Our aim was to verify whether the quantitative determination of PSA mRNA in circulating cells is helpful diagnosing and scoring localized prostate cancer (PC). Design methods: The study included 145 patients with benign prostatic hyperplasia (BPH), 138 PC 28 healthy controls (CS). cDNA amplified by real-time PCR from mononuclear cells. Serum total free were determined. Prostate cancers histologically scored according Gleason criteria. Results: most sensitive index tPSA (70%), specific f/t (80%). High found more frequently poorly differentiated (23.1%) than those well (4.5%) or moderately (4.3%) tumors. Conclusions: are best available tools for discriminating between BPH. assessment blood might be biochemical grading cancer.
core.ac.uk
elsevier.com
sci-hub.se 参考文章(30)
Seth P. Lerner, Carie Seale-Hawkins, C. Eugene Carlton, Peter T. Scardino, The Risk of Dying of Prostate Cancer in Patients with Clinically Localized Disease The Journal of Urology. ,vol. 146, pp. 1040- 1045 ,(1991) , 10.1016/S0022-5347(17)37997-1
Chun-Ling Gao, Sudhir K Rawal, Leon Sun, Amina Ali, Roger R Connelly, Lionel L Banez, Isabell A Sesterhenn, David G Mcleod, Judd W Moul, Shiv Srivastava, None, Diagnostic potential of prostate-specific antigen expressing epithelial cells in blood of prostate cancer patients. Clinical Cancer Research. ,vol. 9, pp. 2545- 2550 ,(2003)
Jean-Luc Gala, Michel Heusterspreute, Sylvain Loric, France Hanon, Bertrand Tombal, Paul Van Cangh, Philippe De Nayer, Marianne Philippe, Expression of prostate-specific antigen and prostate-specific membrane antigen transcripts in blood cells: implications for the detection of hematogenous prostate cells and standardization Clinical Chemistry. ,vol. 44, pp. 472- 481 ,(1998) , 10.1093/CLINCHEM/44.3.472
A. ZIPPELIUS, K. PANTEL, RT-PCR-Based Detection of Occult Disseminated Tumor Cells in Peripheral Blood and Bone Marrow of Patients with Solid Tumors: An Overview Annals of the New York Academy of Sciences. ,vol. 906, pp. 110- 123 ,(2006) , 10.1111/J.1749-6632.2000.TB06600.X
R.A Ghossein, S Bhattacharya, Molecular detection and characterisation of circulating tumour cells and micrometastases in solid tumours. European Journal of Cancer. ,vol. 36, pp. 1681- 1694 ,(2000) , 10.1016/S0959-8049(00)00152-0
S. Lintula, S. Vesalainen, A. Rannikko, W.‐M. Zhang, P. Finne, J. Stenman, U.‐H. Stenman, Quantification of prostate specific antigen mRNA levels in circulation after prostatic surgery and endocrine treatment by quantitative reverse transcription-polymerase chain reaction. Scandinavian Journal of Clinical & Laboratory Investigation. ,vol. 64, pp. 93- 100 ,(2004) , 10.1080/00365510410004191
Stefania Gelmini, Carmela Tricarico, Luisa Petrone, Gianni Forti, Andrea Amorosi, Giovanni L. Dedola, Mario Serio, Mario Pazzagli, Claudio Orlando, Real-time RT-PCR for the measurement of prostate-specific antigen mRNA expression in benign hyperplasia and adenocarcinoma of prostate. Clinical Chemistry and Laboratory Medicine. ,vol. 41, pp. 261- 265 ,(2003) , 10.1515/CCLM.2003.040
Shahrokh F Shariat, Michael W Kattan, Sibel Erdamar, Cuong Nguyen, Peter T Scardino, David M Spencer, Thomas M Wheeler, Kevin M Slawin, None, Detection of clinically significant, occult prostate cancer metastases in lymph nodes using a splice variant-specific rt-PCR assay for human glandular kallikrein. Journal of Clinical Oncology. ,vol. 21, pp. 1223- 1231 ,(2003) , 10.1200/JCO.2003.08.142
Ralf Kurek, Alice Ylikoski, Heiner Renneberg, Lutz Konrad, Gerd Aumüller, Sandra J. Roddiger, Nikolaos Zamboglou, Ulf W. Tunn, Hans Lilja, Quantitative PSA RT-PCR for preoperative staging of prostate cancer. The Prostate. ,vol. 56, pp. 263- 269 ,(2003) , 10.1002/PROS.10257
Shandra S. Wilson, E.David Crawford, Screening for prostate cancer: current recommendations. Urologic Clinics of North America. ,vol. 31, pp. 219- 226 ,(2004) , 10.1016/J.UCL.2004.01.002