Quantification of prostate specific antigen mRNA levels in circulation after prostatic surgery and endocrine treatment by quantitative reverse transcription-polymerase chain reaction.
作者： S. Lintula , S. Vesalainen , A. Rannikko , W.‐M. Zhang , P. Finne
DOI: 10.1080/00365510410004191
关键词:
摘要: Various methods to detect prostatic cells in circulation have given conflicting results. This is probably because qualitative rather than quantitative been used mRNA from cells. A method has developed based on reverse transcription‐polymerase chain reaction (RT‐PCR) for detection of prostate specific antigen (PSA) peripheral blood. competitive internal standard was quantification absolute amounts PSA mRNA. The limit the assay 7 copies mRNA, and highest level circulating 88 control subjects 25 per milliliter study influence surgery endocrine treatment 56 patients undergoing biopsy, radical prostatectomy, transurethral resection (TURP), orchiectomy, or androgen blockade. Blood samples were drawn before, during up 26 weeks after these procedures had carried ...
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