EBNA-5, an Epstein-Barr virus-encoded nuclear antigen, binds to the retinoblastoma and p53 proteins

摘要: Abstract Epstein-Barr virus (EBV) immortalized human lymphoblastoid cell lines express six virally encoded nuclear proteins, designated EBV antigens 1-6 (EBNA-1-6). We show that the EBNA-5 protein (alternatively EBNA-LP) is required for B-cell transformation can form a molecular complex with retinoblastoma (RB) and p53 tumor suppressor proteins. Using deletion mutants, we have found 66-amino acid-long peptide, by W repeat of genome, sufficient binding. Point mutations RB inhibit their complexing other DNA viral oncoproteins do not affect binding to EBNA-5. competes Our data suggest mechanisms involved in may include impairment function.