Wild-Type versus Mutant p53

摘要: What is now known as p53 was initially identified a normal cellular protein bound to SV40 large T antigen.1,2 Immunoprecipitation of antigen from transformed mouse cell line coprecipitated nuclear phosphoprotein 53,000 molecular weight, hence called p53. The human composed 393 amino acids and located in the nucleus. present all tissues but such low quantities3–5 that it difficult detect by immunohistochemical techniques. On other hand, has been detected at much higher levels number sporadic tumors virally chemically-transformed lines mice humans.6,7 Isolation characterization gene followed early transfection studies indicated capable immortalizing primary rat embryonic fibroblast cells culture. It also found could cooperate with activated ras oncogene transformation culture.8–10