Visualisation, by immunocytochemistry, of p53 at the plasma membrane of both nontransformed and SV40-transformed cells

摘要: The cellular protein p53 normally functions in the control of cell proliferation; but, when expressed abnormally also contributes towards process transformation. functioning is thought to involve interaction with specific targets and, SV40- and other transformed cells, located nucleus: thus may function via interacting nuclear components. In addition, there indirect evidence that SV40-transformed cells associated plasma membrane; however, this not evident stained by immunofluorescence. We have sought obtain direct for at membrane staining a very sensitive immunocytochemical procedure. compared BALB 3T3 3T3, NIH transfected v-mos provirus. last line tumorogenic. found each line. nontransformed association was restricted period mitosis. These results indicate normal, rather than transformation-related phenomenon, temporally linked