Larval Exposure to Chlorpyrifos Affects Nutritional Physiology and Induces Genotoxicity in Silkworm Philosamia ricini (Lepidoptera: Saturniidae).

摘要: Chlorpyrifos is a most widely used organophosphate insecticide because of its cost effectiveness and degradable nature. However, this pesticide enters contaminates the environment either by direct application, spray drifts or crop run off shows adverse effect on non-targeted organisms. Philosamia ricini (eri silkworm), one exploited, domesticated commercialized non mulberry silkworm known for mass production eri silk. The larvae get exposed to residues leaves food plants. present study investigates commercial formulation chlorpyrifos (Pyrifos-20 EC) silkworm. Initially LC50 value was determined at 24 - 96 h further experiments were carried out with sub lethal concentrations after exposure period. potential toxicity evaluated as fuction metabolism nutritional physiology in 3rd, 4th 5th instar larvae. Alteration histoarchitecture gut concentration also studied. induced genotoxicity hemocytes investigated single cell gel electrophoresis, micronuclei assay apoptosis assay. Herein, values calculated 3.83, 3.35, 2.68 2.35 mg/L h, 48 72 respectively. A significant decrease trehalose activity along digestive enzyme observed affected groups (P < 0.05). Further, revealed higher tail percentage, length moment damage DNA 0.001). Moreover, 2.0 concentration, ~ 10 fold increases compared control. Results showed activation caspase following hr (1.5 mg/L) dose-dependent manner. control group less number apoptotic cells detected, however both more. Taken together, provides evidence that pollution might have overall could lead reduced survivability economically beneficial