Inhibition of human immunodeficiency virus gene amplification by heparin.

摘要: Gene amplification of virus-specific sequences is widely used as a method to detect or confirm human immunodeficiency virus (HIV) infection. In this study we an enzyme-linked affinity assay quantify polymerase chain reaction products from whole blood, plasma, and separated mononuclear cells collected in the presence four common anticoagulants: acid citrate dextrose, sodium EDTA, potassium oxalate, heparin. Attenuation product signal was observed after nucleic extraction washed cells, plasma specimens These inhibitory effects on gene could be reversed with heparinase. The addition little 0.05 U heparin completely inhibited HLA-DQa sequence placental DNA. We conclude that can cause attenuation inhibition amplification. Acid dextrose which lack activity, are most appropriate anticoagulants for clinical blood samples when anticipated. Images