Inhibition of growth and squamous‐cell differentiation markers in cultured human head and neck squamous carcinoma cells by β‐all‐trans retinoic acid

摘要: Vitamin A and some of its metabolites such as beta-all-trans retinoic acid (RA) have been implicated in the regulation differentiation normal malignant epithelial cells vivo vitro. In present study effects RA on growth 7 cell lines derived from human head neck squamous-cell carcinomas (HNSCCs) were examined. (greater than 0.01 microM) inhibited proliferation monolayer culture 6 HNSCC lines. One line (UMSCC-35) was very sensitive, 5 (UMSCC-10A, -19, -30, -22B 1483) moderately 1 (HNSCC 183) insensitive. Three (UMSCC-22B, capable forming colonies semisolid medium--a capability that suppressed by RA. The expressed various levels markers type I (particulate, epidermal) transglutaminase (TGase) cholesterol sulfate (CS). treatment (I microM, days) decreased TGase activity more 50% 3 lines, effect UMSCC-22B dose-dependent. Type II (soluble, tissue type) detected after increased 1483 183 UMSCC-19. Following treatment, CS 20, 25, 70, 76, 89 91% UMSCC-30, -10A, 183, UMSCC-35, -22B, 1483, respectively. suppression accumulation Cholesterol sulfotransferase activity, which is responsible for synthesis, 40-97% UMSCC-19, 1483. Our results demonstrate inhibits decreases level 2 squamous cells.