ESEM-EDS: In vivo characterization of the Ni hyperaccumulator Noccaea caerulescens.

摘要: Environmental scanning electron microscopy (ESEM) permits to analyze samples in their native-hydrated state, allowing a broad spectrum of biological applications. In this study, ESEM equipped with energy dispersive X-ray spectrometer (EDS) was used as fast method tissue morphology and investigate metal distribution the Ni hyperaccumulator Noccaea caerulescens, an established model study adaptation plants metalliferous soils. The low vacuum wet mode operative conditions required proper choice experimental parameters both for morphological compositional characterization plant tissues. calibration strategy semi-quantitative analysis involved use fortified agar standard signal normalization respect endogenous carbon, chosen internal standard. obtained results are accordance present literature, showing preferential epidermal cells near stomata leaves cotyledon epidermidis parenchyma area seeds. Thanks absence any time consuming sample treatment steps, ESEM-EDS technique can be proposed valid vivo high-throughput tissues rapid screening identification other selected contaminated area.