Quantitative X-ray microanalysis of biological specimens

摘要: Qualitative X-ray microanalysis of biological specimens requires an approach that is somewhat different from used in the materials sciences. The first step deconvolution and background subtraction on obtained spectrum. further treatment depends type specimen: thin, thick, or semithick. For thin sections, continuum method quantitation most often used, but it should be combined with accurate correction for extraneous background. However, alternative methods to determine local mass also considered. In analysis bulk specimens, ZAF-correction appears less useful, primarily because uneven surface specimens. peak-to-local model may a more adequate thick are not mounted substrate. Quantitative generally use standards preferably resemble specimen chemical physical properties. Special problems include low count rates, instability loss, contributions spectrum, preparative artifacts affecting quantitation. A relatively recent development quantitative determination water content.