Oxygen supply to immobilized cells: 5. Theoretical calculations and experimental data for the oxidation of glycerol by immobilized Gluconobacter oxydans cells with oxygen or p-benzoquinone as electron acceptor.

摘要: Theoretical calculations of reaction kinetics were done for one-step reactions catalyzed by cells immobilized in spherical beads. The free assumed to obey Michaelis-Menten a one-substrate reaction. Both external (outside the beads) and internal (inside mass transfer substrate considered preparations. theoretical compared with experimental data oxidation glycerol dihydroxyacetone Gluconobacter oxydans calcium alginate gel. Glycerol was present excess so that rate limited oxygen. correlation between quite good. showed how overall effectiveness factor influenced by, example, particle size cell density In most cases mainly (oxygen). As shown previously, p-benzoquinone can replace oxygen as electron acceptor this same equations used rate-limiting substrate. Parameters such diffusivity, maximal rate, K were, course, different. case also, model results Much higher production rates obtained when used. reasons fact gave solubility than Different methods increasing microbial are discussed.