BIM induction of apoptosis triggered by EGFR-sensitive and resistance cell lines of non-small-cell lung cancer
作者： Zhanxia Li , Songwen Zhou , Ling Zhang , Chunxia Su , Jinqin Hang
DOI: 10.1007/S12032-010-9470-Y
关键词: Flow cytometry 、 Cell killing 、 Tyrosine kinase 、 Cisplatin 、 MTT assay 、 Cell biology 、 Biology 、 Cell culture 、 Apoptosis 、 Gefitinib
摘要: We sought to improve the understanding of oncogene-dependent and independent non-small-cell lung cancer (NSCLC), which could provide insight into mechanism sensitivity and/or resistance tyrosine kinase inhibitors or chemotherapeutics. NSCLC cell lines with different EGFR genotypes were used in this study; MTT assay flow cytometry applied study sensitivities these gefitinib cisplatin. Western blot was performed determine expression levels BIM other Bcl-2 family proteins pre- pro-treatment. Gefitinib provoked apoptosis caspase activation via intrinsic pathways significantly up-regulated protein drug-sensitive PC-9 line, but not resistant PC-9/BB4 line. The knockdown by RNA interference virtually eliminated gefitinib-induced killing cells vitro. Cisplatin induce lines, including H1299, A549, PC-9, cells, associated overexpression BIM. is involved TKI-induced sensitive EGFR-mutant Down-regulation both seen acquired induction may have a role treatment TKI-resistant tumors.
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