Microarray expression profile of long non-coding RNAs in EGFR-TKIs resistance of human non-small cell lung cancer.

摘要: The application of epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) is limited by drug resistance in non-small cell lung cancer (NSCLC). Long non-coding RNAs (lncRNAs) are known to be involved tumor development and metastasis, as well chemotherapy resistance. To gain insight into the molecular mechanisms EGFR-TKIs resistance, EGFR-TKIs‑sensitive ‑resistant human cells were analyzed lncRNA microarray. In present study, we found a total 22,587 lncRNAs expressed cells. Of these, expression level 1,731 was upregulated >2-fold compared with gefitinib-sensitive while that 2,936 downregulated. Bioinformatics analysis (GO pathway analyses) revealed some classical pathways participating proliferation apoptosis aberrantly these (P-value cut-off 0.05). Enhancer-like their nearby coding genes analyzed. Six identified potential enhancers. Several validated lines using RT-qPCR. best our knowledge, results showed for first time differentially responded NSCLC LncRNAs may therefore novel candidate biomarkers targets therapy future.