Evaluation of Brassica rapa L. genotypes for microspore culture response and identification of a highly embryogenic line.
作者： A.M.R. Ferrie , D.J. Epp , W.A. Keller
DOI: 10.1007/BF00231942
关键词: Brassica 、 Gametophyte 、 Ploidy 、 Biology 、 Botany 、 Brassica rapa 、 Microspore 、 Cultivar 、 Doubled haploidy 、 Genotype
摘要: Isolated microspore culture techniques are being widely used in Brassica breeding programs to generate haploid and doubled plants. A number of factors influence regeneration response vitro including genotype. In order assess the effect genotype on embryogenesis B. rapa L. var. oleifera, 17 cultivars lines were evaluated. Embryos developed from all but one when using NLN medium with 17% sucrose, followed by a reduction sucrose concentration 10%, 48 h later. The embryos /100 buds differed between genotypes, ranging 0 70. Further studies indicated that incubation time influenced embryogenesis. Selection carried out an Agriculture Agri-Food Canada line have resulted identification highly embryogenic line. This produced thousands microspore-derived will be useful mutant selection gene transfer as well biochemical developmental studies.
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