Measurement of subcellular texture by optical Gabor-like filtering with a digital micromirror device

摘要: We demonstrate an optical Fourier processing method to quantify object texture arising from subcellular feature orientation within unstained living cells. Using a digital micromirror device as spatial filter, we measured cellular responses two-dimensional Gabor-like filters optimized sense of nonspherical particles, such mitochondria, with width around 0.45 microm. Our showed significantly rounder structures apoptosis-defective cells lacking the proapoptotic mitochondrial effectors Bax and Bak, when compared Bax/Bak expressing functional for apoptosis, consistent reported differences in shape these By decoupling frequency resolution image resolution, this enables rapid analysis submicrometer scatterers undersampled large field view yields spatially localized morphometric parameters that improve quantitative assessment biological function.