Spatiotemporal Control of Intracellular Phase Transitions Using Light-Activated optoDroplets
作者： Yongdae Shin , Joel Berry , Nicole Pannucci , Mikko P. Haataja , Jared E. Toettcher
DOI: 10.1016/J.CELL.2016.11.054
关键词: Intracellular 、 Phase transition 、 Biology 、 RNA-binding protein 、 Biophysics 、 Bioinformatics 、 Optogenetics 、 Organelle 、 RNA 、 Light activated 、 Phase boundary 、 General Biochemistry, Genetics and Molecular Biology
摘要: Phase transitions driven by intrinsically disordered protein regions (IDRs) have emerged as a ubiquitous mechanism for assembling liquid-like RNA/protein (RNP) bodies and other membrane-less organelles. However, a lack of tools to control intracellular phase transitions limits our ability to understand their role in cell physiology and disease. Here, we introduce an optogenetic platform that uses light to activate IDR-mediated phase transitions in living cells. We use this “optoDroplet” system to study condensed phases driven by the IDRs of …
princeton.edu
mendeley.com
unirioja.es
elsevier.com参考文章(58)
M. Th. van Genuchten, W. J. Alves, Analytical solutions of the one-dimensional convective-dispersive solute transport equation United States. Dept. of Agriculture. Technical bulletin (USA). ,(1982) , 10.22004/AG.ECON.157268
Robert D. Phair, Tom Misteli, High mobility of proteins in the mammalian cell nucleus Nature. ,vol. 404, pp. 604- 609 ,(2000) , 10.1038/35007077
Yuan Lin, David S.W. Protter, Michael K. Rosen, Roy Parker, Formation and Maturation of Phase-Separated Liquid Droplets by RNA-Binding Proteins Molecular Cell. ,vol. 60, pp. 208- 219 ,(2015) , 10.1016/J.MOLCEL.2015.08.018
Sonja Kroschwald, Shovamayee Maharana, Daniel Mateju, Liliana Malinovska, Elisabeth Nüske, Ina Poser, Doris Richter, Simon Alberti, Promiscuous interactions and protein disaggregases determine the material state of stress-inducible RNP granules eLife. ,vol. 4, ,(2015) , 10.7554/ELIFE.06807
Kathleen A. Burke, Abigail M. Janke, Christy L. Rhine, Nicolas L. Fawzi, Residue-by-Residue View of In Vitro FUS Granules that Bind the C-Terminal Domain of RNA Polymerase II Molecular Cell. ,vol. 60, pp. 231- 241 ,(2015) , 10.1016/J.MOLCEL.2015.09.006
Avinash Patel, Hyun O Lee, Louise Jawerth, Shovamayee Maharana, Marcus Jahnel, Marco Y Hein, Stoyno Stoynov, Julia Mahamid, Shambaditya Saha, Titus M Franzmann, Andrej Pozniakovski, Ina Poser, Nicola Maghelli, Loic A Royer, Martin Weigert, Eugene W Myers, Stephan Grill, David Drechsel, Anthony A Hyman, Simon Alberti, None, A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation Cell. ,vol. 162, pp. 1066- 1077 ,(2015) , 10.1016/J.CELL.2015.07.047
Joel Berry, Stephanie C. Weber, Nilesh Vaidya, Mikko Haataja, Clifford P. Brangwynne, RNA transcription modulates phase transition-driven nuclear body assembly Proceedings of the National Academy of Sciences. ,vol. 112, pp. E5237- E5245 ,(2015) , 10.1073/PNAS.1509317112
Frédéric Cardinaux, Thomas Gibaud, Anna Stradner, Peter Schurtenberger, Interplay between Spinodal Decomposition and Glass Formation in Proteins Exhibiting Short-Range Attractions Physical Review Letters. ,vol. 99, pp. 118301- ,(2007) , 10.1103/PHYSREVLETT.99.118301
Stephanie C. Weber, Clifford P. Brangwynne, Inverse Size Scaling of the Nucleolus by a Concentration-Dependent Phase Transition Current Biology. ,vol. 25, pp. 641- 646 ,(2015) , 10.1016/J.CUB.2015.01.012
Matthew J Kennedy, Robert M Hughes, Leslie A Peteya, Joel W Schwartz, Michael D Ehlers, Chandra L Tucker, Rapid blue-light-mediated induction of protein interactions in living cells Nature Methods. ,vol. 7, pp. 973- 975 ,(2010) , 10.1038/NMETH.1524