High mobility of proteins in the mammalian cell nucleus

摘要: The mammalian cell nucleus contains numerous sub-compartments, which have been implicated in essential processes such as transcription and splicing. mechanisms by nuclear compartments are formed maintained unclear. More fundamentally, it is not known how proteins move within the nucleus. We measured kinetic properties of living cells using photobleaching techniques. Here we show that involved diverse rapidly throughout entire Protein movement independent energy, indicates may use a passive mechanism movement. Proteins associate dissociate with compartments. Using modelling, determined residence times steady-state fluxes molecules two main These data many roam vivo reflection association/dissociation its 'residents' nucleoplasmic space. Our observations conceptual implications for understanding architecture organized vivo.