Dynamic relocation of chromosomal protein HMG-17 in the nucleus is dependent on transcriptional activity.

摘要: Chromosomal proteins HMG-14/-17 are nucleosomal binding proteins, which alter the structure of chromatin fiber and enhance transcription, but only from templates. Here we show that in tissue culture cells, HMG-17 protein colocalizes with sites active transcription. Incubation permeabilized cells a peptide corresponding to domains specifically arrested polymerase II-dependent In these displaces reduces cellular content protein. These results suggest presence is required for efficient II non-permeabilized, actively transcribing dispersed punctate pattern, throughout nucleus. Upon transcriptional inhibition by alpha-amanitin or actinomycin D, gradually redistributes until it localizes fully interchromatin granule clusters, together splicing factor SC35. The association dynamic rather than static, absence released accumulates clusters. Thus, intranuclear distribution chromosomal act as architectural elements may be functionally related activity cell.