Millimolar concentrations of ascorbic acid in purified human mononuclear leukocytes. Depletion and reaccumulation.
作者： P Bergsten , G Amitai , J Kehrl , K R Dhariwal , H G Klein
DOI: 10.1016/S0021-9258(19)39841-2
关键词: Chromatography 、 Vitamin C 、 Ascorbic acid 、 Molar concentration 、 Extracellular 、 Intracellular 、 Quantitative analysis (chemistry) 、 Cell culture 、 Lymphocyte 、 Biochemistry 、 Chemistry
摘要: Ascorbic acid (vitamin C) was found in isolated human mononuclear leukocytes and their purified components millimolar concentration. Intracellular ascorbic depleted greater than 96% during cell culture rapidly reaccumulated after addition of physiologic concentrations to the extracellular medium. Purified cells maintained concentration gradients as large 100-fold across plasma membrane. The ability vary intracellular over such a wide range makes it possible for first time these study function direct relationship
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