Deciphering the genetic and transcriptional basis of cross-stress responses in Escherichia coli under complex evolutionary scenarios

摘要: A tantalizing question in microbial physiology is the inter-dependence and evolutionary potential of cellular stress response across multiple environmental dimensions. To address this question, we comprehensively characterized cross-stress behavior wild-type evolved Escherichia coli populations five abiotic stresses (n-butanol, osmotic, alkaline, acidic, oxidative) by performing genome-scale genetic, transcriptional growth profiling, thus identifying 18 cases protection one case vulnerability. We identified vulnerability, along with core stress-specific networks. tested several hypotheses regarding effect order, combinations, mutation reversal varying environments to evolution final fitness respective populations. Our results argue a common systems-level crucial implicated pathways that include metal ion binding glycolysis/gluconeogenesis further complemented expression program.