Comparative assessment of albendazole and triclabendazole ovicidal activity on Fasciola hepatica eggs

摘要: The benzimidazole compounds albendazole (ABZ) and triclabendazole (TCBZ) are both effective against Fasciola hepatica, although ABZ is only adult flukes. Additionally, a broad-spectrum nematodicidal compound with well-known ovicidal activity. However, no data on the effect of TCBZ F. hepatica eggs available. work reported here evaluated comparative ABZ, their sulphoxide metabolites recovered from bile sheep artificially infected either TCBZ-susceptible (Cullompton) or TCBZ-resistant (Sligo) isolate hepatica. effects different non-flukicidal methylcarbamate hatching were evaluated. Eggs (500 eggs/mL, n=4) incubated for 12h TCBZ, (TCBZ.SO), (5, 10 20 nmol/mL) without drug (untreated control) (Experiment 1). TCBZ.SO (5 egg hatchability was examined after long (15 days) exposure 2). Furthermore, ABZ.SO at concentrations 1, 0.5, 0.1 0.05 3), fenbendazole (FBZ), oxfendazole (OFZ), mebendazole (MBZ), flubendazole (FLBZ) reduced-FLBZ (R-FLBZ) (2 microg/mL) fluke eggs, 12-h 4). Egg hatch assessed by direct microscopic observation incubation 25 degrees C 15 days. did not affect incubation. A similar result obtained much longer 1 significant (P<0.05) inhibition observed in ABZ- ABZ.SO-incubated (Experiments 3). 4) affected hatchability, particularly FLBZ R-FLBZ. In conclusion, had clear inhibitory development most extensively used flukicidal compound, its main metabolite, hatch, even