Screening anthelmintic resistance to triclabendazole in Fasciola hepatica isolated from sheep by means of an egg hatch assay
作者： David Robles-Pérez , José Manuel Martínez-Pérez , Francisco Antonio Rojo-Vázquez , María Martínez-Valladares
DOI: 10.1186/S12917-015-0543-1
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摘要: Background In the present study, detection of anthelmintic resistance to triclabendazole (TCBZ) in sheep infected by Fasciola hepatica was studied using an egg hatch assay (EHA). F. eggs were recovered from bile and faeces animals isolates with different grade TCBZ: i) a resistant isolate (RT); ii) susceptible (ST); iii) naturally field strain (FST). In EHA percentage hatched calculated according following concentrations TCBZ diluted dimethyl-sulfoxide (DMSO): 0.05, 0.2, 1, 5, 25 μg/ml.
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