Long non‐coding ribonucleic acid zinc finger antisense 1 promotes the progression of colonic cancer by modulating ZEB1 expression

摘要: Background and aim Long non-coding RNA ZFAS1 (zinc finger antisense 1) is frequently amplified in hepatocellular carcinoma promotes metastasis by increasing zinc E-box binding homeobox 1 (ZEB1), which can potentiate the progression of epithelial-to-mesenchymal transition (EMT). However, expression pattern role colonic cancer remains unknown. The present study aimed to investigate its clinical significance cancer. Methods Paired tissue samples clinicopathologic characteristics 73 patients were analyzed. Quantitative real-time PCR analysis was used evaluate levels tissues, cell lines plasma. ZEB1 EMT-related markers also explored. Cell biology assays explore biologic consequences regulating proliferation invasion, as well roles EMT. Results ZFAS1 up-regulated tissues compared with adjacent mucosa (p < 0.01), level significantly correlated TNM stage, vascular invasion lymph node (p < 0.05). increased patients’ Moreover, promoted proliferation, impeded apoptosis. Knockdown decreased epithelial E-cadherin, ZO-1 while decreasing mesenchymal vimentin N-cadherin. Conclusions LncRNAZFAS1 may function an oncogene modulating induce EMT. Manipulation be a novel approach suppress progression. In addition, plasma has potential diagnostic biomarker cancer.