Molecular identification of anisakid nematodes third stage larvae isolated from common squid (Todarodes pacificus) in Korea

作者: Eko Setyobudi , Chan-Hyeok Jeon , Kwangho Choi , Sung Il Lee , Chung Il Lee

DOI: 10.1007/S12601-013-0016-Z

关键词: Todarodes pacificusBody cavityHost (biology)ZoologyLarvaFisherySpecies distributionBiologyAnisakis simplexSquidAnisakisOceanography

摘要: The occurrence of Genus Anisakis nematode larvae in marine fishes and cephalopods is epidemiologically important because simplex larval stage can cause a clinical disease humans when infected hosts are consumed raw. Common squid (Todarodes pacificus) from Korean waters were investigated for anisakid nematodes infection during 2009∼2011. In total, 1,556 collected 615 common squids 732 them subsequently identified by PCR-RFLP analysis ITS rDNA. Depending on the sampling locations, showed different prevalence, intensity species distribution. A high prevalence (P) mean (MI) observed Yellow Sea (n = 250, P 86.0%, MI 5.99 larvae/host) southern sea Korea 126, 57.1%, 3.36 larvae/host). pegreffii was dominantly found (127/ 140, 90.7%) (561/565, 98.9%). contrast, relatively low East 239, 8.37%, 1.25 A. not 52.0% (13/25) as simplex. Most body cavity or digestive tract squid, which rarely raw humans. Considering differenences distribution their microhabitat it remains unclear whether plays an role epidemiology human anisakis Korea. Further extensive identification with geographical seasonal information will be necessary.

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