Occurrence of anisakid nematode larvae in chub mackerel (Scomber japonicus) caught off Korea.

摘要: Chub mackerel (Scomber japonicus) is a pelagic fish species widely distributing in the Indo-Pacific and commercially important Korea. It known to harbor anisakid nematodes larvae, ingesting raw or undercooked can accidentally cause human infection. In this study, we isolated nematode larvae 417 chub caught from 7 sampling locations around Korean Peninsula 2011 2012, identified them by PCR-RFLP of ITS (internal transcribed spacer) ribosomal DNA direct sequencing mitochondrial cox2 gene. The prevalence infection was 55.4% (231/417) mean intensity 7.0 (1628/231). Most (1523/1628; 93.6%) were found body cavity, while 5.5% (89/1628) gastrointestinal tract. Four different sequencing. (1535/1628; 94.3%) as Anisakis pegreffii, 2.8% (46/1628) Hysterothylacium sp. A hybrid genotype (Anisakis simplex sensu stricto×A. pegreffii) A. stricto 2.5% (41/1628) 0.4% (6/1628) nematodes, respectively. assemblage Korea similar that Tsushima Current stock Japan, pegreffii dominant species. Since most cavity sequencing, may not greatly contribute anisakidosis Alternately, be responsible for Korea, addition stricto. Further studies, such molecular diagnosis anisakidosis, are necessary assessing epidemiological role