Development of a novel adenovirus–alphavirus hybrid vector with RNA replicon features for malignant hematopoietic cell transduction
作者： Y Yang , F Xiao , Z Lu , Z Li , H Zuo
DOI: 10.1038/CGT.2013.37
关键词: Hybrid vector 、 Semliki Forest virus 、 K562 cells 、 Biology 、 Leukemia 、 Molecular biology 、 Replicon 、 Cell culture 、 Jurkat cells 、 Virology 、 Multiplicity of infection 、 Molecular medicine 、 Cancer research
摘要: To improve the expression levels of transgenes in malignant hematopoietic cells, we developed a novel adenoviral-alphavirus hybrid vector Ad5/F11p-SFV-GFP that contains Semliki Forest Virus (SFV) replicon and chimeric fibers Ad5 Ad11p. infected >95% K562, U937 or Jurkat cells 23.65% HL-60 led to moderate Enhanced Green Fluorescent Protein (EGFP) transgene intensity. The infection efficiency primary human leukemia ranged from 9.34-89.63% (median, 28.58%) at multiplicity (MOI) 100, compared with only 3.37-44.54% 10.42%) by Ad5/F11p-GFP. Importantly, significantly higher level as indicated relative fluorescence intensity, increased EGFP Ad5/F11p-SFV-GFP-infected was associated accumulation abundant subgenomic mRNA. Additionally, inhibited blocking CD46 receptor; however, other factors may affect gene-transfer cells. In conclusion, successfully RNA features, which represents promising for gene modifications during production cell-based vaccines patients.
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