The Isolation of Histone from Neurospora crassa
作者： Myrtle W. Hsiang , R. David Cole
DOI: 10.1016/S0021-9258(19)44179-3
关键词: Crassa 、 Nucleosome 、 Biochemistry 、 Neurospora crassa 、 DNA 、 Molecular mass 、 Histone 、 Histone octamer 、 Biology 、 Chromatin
摘要: Abstract Two slightly lysine-rich histones (Fractions C-3 and C-4) have been isolated from Neurospora crassa chromatin during exponential growth. Their molecular weights are 14,000 8,000, respectively. Fraction is similar to the histone F2b of calf thymus in weight, amino acid composition, disc gel electrophoretic mobility, chromatographic behavior on Bio-Gel P-10. C-4 resembles other F2a2 but size much smaller than higher organisms. N. contains RNA, histone, non-histone proteins; amounts these components relative DNA about 0.14, 0.24, 0.60 (by weight), This amount one-quarter usually found eukaryotes.
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