Identification of a fungal community on gilded wood carved heritage
作者： Ana Catarina Pinheiro , Bruna P. Oliveira , Cristina Veríssimo , João C. Brandão , Laura Rosado
DOI: 10.1016/J.CULHER.2012.03.008
关键词: Engineering 、 Identification (biology) 、 Botany 、 Archaeology 、 Cultural heritage 、 Fungal DNA 、 Paecilomyces lilacinus 、 Antifungal 、 Serpula lacrymans
摘要: Abstract During restoration activities, a fungal reddish area was noted on gilded wood carved ceiling decoration from sacristy of church in Aveiro, Portugal. The protocols used to identify the community present this wooden material included classical culturing methods and molecular biology protocol based ability denaturing high performance liquid chromatography (DHPLC) separate DNA several species. This last method complemented results obtained with traditional approach overall study revealed three species: Serpula lacrymans (Wulfen) P. Karsten, Paecilomyces lilacinus (Thom) Samson Penicillium chrysogenum Thom. is, author's knowledge, first documented case S. Portuguese built cultural heritage. Despite antifungal solution applied dryer environment recommended, water activity (Aw) levels recorded location are still dangerously may allow regrowth. Periodic inspections were advised.
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