Association of a lipoprotein-like particle with bovine fetuin.
作者： Lakshmikantha Kumbla , Antonio J. Cayatte , M. T. Ravi Subbiah
DOI: 10.1096/FASEBJ.3.9.2472993
关键词: High-density lipoprotein 、 Cholesteryl ester 、 Cholesterol 、 High-performance liquid chromatography 、 Agarose 、 Chemistry 、 Chromatography 、 Gel electrophoresis 、 Fetuin 、 Gel permeation chromatography
摘要: Fetuin is a major protein of fetal bovine serum that exhibits heterogeneity and has been found to be associated with some biological active growth factors. Preliminary studies indicated commercial fetuin preparations contain lipids. We investigated in detail the nature lipids by using ultracentrifugation agarose gel chromatography followed lipid analysis. was variety lipids, predominantly cholesterol, cholesteryl ester smaller amounts phospholipids, triglycerides, free fatty acids. Adjustment preparation for various densities resulted fraction density 1.063-1.21 g/ml (1-2% total protein) contained bulk This eluted as single peak upon high pressure liquid chromatography. Delipidation lipoprotein-like particle sodium dodecyl sulfate-polyacrylamide electrophoresis band range itself. These suggest (or isoform) binds forming floating characteristics similar lipoproteins.
fasebj.org 参考文章(16)
H.F. Deutsch, FETUIN: THE MUCOPROTEIN OF FETAL CALF SERUM Journal of Biological Chemistry. ,vol. 208, pp. 669- 678 ,(1954) , 10.1016/S0021-9258(18)65593-0
Alan D. Cardin, George Holdsworth, Richard L. Jackson, Isolation and Characterization of Plasma Lipoproteins and Apolipoproteins Methods in pharmacology. ,vol. 5, pp. 141- 166 ,(1984) , 10.1007/978-1-4684-4778-1_11
Robert G. Spiro, Studies on Fetuin, a Glycoprotein of Fetal Serum I. ISOLATION, CHEMICAL COMPOSITION, AND PHYSIOCOCHEMICAL PROPERTIES Journal of Biological Chemistry. ,vol. 235, pp. 2860- 2869 ,(1960) , 10.1016/S0021-9258(18)64553-3
S. P. Bydlowski, R. L. Yunker, M. T. Subbiah, Ontogeny of 6-keto-PGF1 alpha synthesis in rabbit aorta and the effect of premature weaning. American Journal of Physiology-heart and Circulatory Physiology. ,vol. 252, ,(1987) , 10.1152/AJPHEART.1987.252.1.H14
Raymond F. Chen, Removal of Fatty Acids from Serum Albumin by Charcoal Treatment Journal of Biological Chemistry. ,vol. 242, pp. 173- 181 ,(1967) , 10.1016/S0021-9258(19)81445-X
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
K.M. Dziegielewska, G. Kocsis, N.R. Saunders, Identification of fetuin and other proteins in cerebrospinal fluid and plasma of fetal pigs during development Comparative Biochemistry and Physiology B. ,vol. 66, pp. 535- 541 ,(1980) , 10.1016/0305-0491(80)90245-X
M T Subbiah, D Deitemeyer, R L Yunker, Decreased atherogenic response to dietary cholesterol in pigeons after stimulation of cholesterol catabolism in early life. Journal of Clinical Investigation. ,vol. 71, pp. 1509- 1513 ,(1983) , 10.1172/JCI110906
K.M Dziegielewska, E Bock, M.E.P Cornelis, K Møllgård, H New, N.R Saunders, Identification of fetuin in human and rat fetuses and in other species Comparative Biochemistry and Physiology Part A: Physiology. ,vol. 76, pp. 241- 245 ,(1983) , 10.1016/0300-9629(83)90321-3
M.C. Park, A. Cayatte, M.T.R. Subbiah, Isolation and characterization of a novel lipoprotein particle from human placental extracts Biochemical and Biophysical Research Communications. ,vol. 153, pp. 502- 509 ,(1988) , 10.1016/S0006-291X(88)81123-9